Sequencer

Trade Agreement: NAFTA/AIT/Canada FTAs with Peru/Colombia/Panama Tendering Procedures: All interested suppliers may submit a bid Attachment: None Competitive Procurement Strategy: Best Overall Proposal Comprehensive Land Claim Agreement: No Nature of Requirements: Sequencer

KW405-140481/A Armstrong, Wendy Telephone No. - (604) 775-7691 Fax No. - (604) 775-7526

Requirement The Toxicogenomic Laboratory in the Pacific and Yukon Laboratory for Environmental Testing, located at the Pacific Environmental Science Centre in North Vancouver, is seeking a benchtop Next Generation DNA Sequencer for three primary applications: 1- To sequence PCR amplicons for forensic DNA species identification (DNA barcoding). These amplicons will be from a varied collection of non-model plant and animal species which may or may not have a reference genome. 2- To identify and sequence microsatellites (also known as short tandem repeats (STR)) and single nucleotide polymorphisms (SNPs) for individual identification of various animal species and determination of geographic origin. Genetic material will originate from non-model animal species. 3- To sequence mRNA transcripts (RNA-seq) for gene expression studies which examine the impact of toxicants on the genes of exposed animals. Genetic material will originate from non-model animal species which may or may not have a reference genome.

Trade Agreements The requirement is subject to the provisions of the North American Free Trade Agreement (NAFTA), and the Agreement on Internal Trade (AIT)." Delivery Date: Above-mentioned

The Crown retains the right to negotiate with suppliers on any procurement.

Documents may be submitted in either official language of Canada.