Sciex QTRAP 6500+ System

Trade Agreement: WTO-AGP/NAFTA/AIT/Canada FTAs with Peru/Colombia/Panama Tendering Procedures: Generally only one firm has been invited to bid Non-Competitive Procurement Strategy: Exclusive Rights Comprehensive Land Claim Agreement: No Vendor Name and Address: AB Sciex LP 71 Four Valley Dr Concord Ontario Canada L4K4V8 Nature of Requirements:

Sciex QTRAP 6500+ System

H5337-164141/A Hooper, Marlyn Telephone No. - (613) 219-8478 ( ) Fax No. - (819) 956-3814 ( )

1. Advance Contract Award Notice (ACAN)

An ACAN is a public notice indicating to the supplier community that a department or agency intends to award a contract for goods, services or construction to a pre-identified supplier, thereby allowing other suppliers to signal their interest in bidding, by submitting a statement of capabilities. If no supplier submits a statement of capabilities that meets the requirements set out in the ACAN, on or before the closing date stated in the ACAN, the contracting officer may then proceed with the award to the pre-identified supplier.

2. Definition of the requirement

Health Canada (HC) requires the supply, delivery and installation of an ultra-high performance liquid chromatography, and a hybrid triple quadrupole/linear ion trap (UHPLC-QTRAP) system to the Pest Management Regulatory Agency (PMRA) Laboratory Services Section(LSS). This requirement include 2 days on-site training for up to five (5) users and software upgrade for five (5) years.

The system must have capabilities of accurately quantifying and unambiguously identifying over 350 different pesticides and otherchemical contaminants, as well as differentiating isomers as low as 100 parts-per-trillion (ppt) concentration levels in both positive and negative modes with fast (less than 5 ms) data acquisition speed and polarity switching in a single analysis.

3. Criteria for assessment of the Statement of Capabilities

Minimum Essential Requirements: Any interested supplier must demonstrate by way of a statement of capabilities that its product meets the following requirements:

Minimum Mandatory Capabilities/Technical/Performance Criteria/Specifications: 1.1. UHPLC-QTRAP System Capabilities 1.1.1. The whole required system must include an ultra-high performance liquid chromatography and a hybrid triple quadrupole/linear ion trap (UHPLC-QTRAP). The UHPLC-QTRAP system must have capabilities of performing MRM (multiple reaction monitoring) for quantification, carrying on enhanced product-ion spectral scan for identification in a single injection for the determination of pesticides and other chemical contaminants in complex samples such as plant leaves and soil. All these must be achieved at 100 parts-per-Trillion (ppt) concentration levels in both positive and negative modes with fast (5 ms) data acquisition speed and polarity switching in a single analysis. The mass spectrometer must be able to perform MRM and enhanced product ion spectral scan simultaneously. As MRM transitions are positively detected, the data files (i.e. product ion spectra) can be directly searched against a mass spectral library to unambiguously confirm the eluted analytes by the product ion spectral scan simultaneous to the quantification being reported in the same injection. The UHPLC-QTRAP must be able amenable to MRM analysis with fast (1 ms) dwell times, in methods with polarity switching, to perform functions of differentiating isomers and separating analytes of interest from interferences in complex vegetation sample matrices. 1.1.2. The UHPLC-QTRAP must have complete capability and compatibility to import and run the existing validated 3200QTRAP LC/MS/MS methods and compound libraries of a large number of pesticides, i.e. ~ 350, already in use in the Quality System at the PMRA Laboratory. As a part of mandatory requirements, all current UHPLC/ESI-MS/MS methods of up to 350 pesticides and libraries are required to transfer from the 3200QTRAP LC/MS/MS to UHPLC-QTRAP in less than 5 mins without retuning instrument parameters for individual pesticides while achieving the sensitivity and a dynamic range specified below. 1.1.3. UHPLC-QTRAP must deliver an increase in sensitivity of at least one hundred (100) times in S/N on UHPLC/MS/MS methods already optimized on the 3200QTRAP LC/MS/MS System for 90% of the 350 pesticides with an extended dynamic quantification range of three (3) orders of magnitude. It must provide the capability of combining positive and negative mode analysis into a single injection method with a 1 ms pause time, 1 ms dwell time and 5 ms polarity switches for a fast data acquisition to increasethe method capacity. The software that comes with UHPLC-QTRAP must enable to directly access and analyse historic archived 3200QTRAP LC/MS/MS data in the Quality System at the PMRA Laboratory. 1.2. Mass Spectrometer 1.2.1. MS type: hybrid triple quadrupole/linear ion trap LC/MS/MS mass spectrometer. 1.2.2. Supplied with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). The instrument ionization source housing must have a source housing hosting interchangeable APCI (Atmospheric Pressure Chemical Ionization) probe and ESI probes. Each probe will be coded for automatic identification by the software/firmware of the host system. 1.2.3. The source must have flow rates compatibility from 5 to 3000 µL/min, without flow splitting for ESI and 200 to 3000 µL /min for APCI. 1.2.4. Sensitivity MRM mode positive: Reserpine 1 pg on column S/N > 200,000. 1.2.5. Sensitivity MRM mode negative: Chloramphenicol 1 pg on column S/N > 80,000. 1.2.6. Triple quad scan speed: 12,000 Da/sec. 1.2.7. Linear ion trap scan speed: 20,000 Da/sec. 1.2.8. Polarity switching time: 5 msec, in MRM and Scheduled MRM (sMRM) modes. 1.2.9. Minimum MRM dwell time: 1 msec. 1.2.10. Triple quad mass range: (m/z) 5-2000. 1.2.11. Linear ion trap mass range: (m/z) 50-2000. 1.2.12. Triple quadrupole mass stability: 0.1 Da over 24 hours. 1.2.13. Scan types: Full scan MS and selected ion for both Q1 and Q3, product ion scan, multiple reaction monitoring (MRM), Scheduled MRM (sMRM), enhanced MS scan, enhanced product ion scan, enhanced resolution scan, MS3 scan, MRM3 scan, and TripleTrap scan modes. 1.2.14. Sensitivity EPI (enhanced product ion scan) mode: Reserpine 50 fg on column 1000 µL/min, 150-650 Da at 10,000 Da/sec, sum of product ions 195 and 174, S/N > 200 and C.V. 150 and C.V.