Robotic Liquid Handler

Trade Agreement: CETA / WTO-AGP / NAFTA / CFTA / FTAs with Peru / Colombia / Panama / Korea Tendering Procedures: Generally only one firm has been invited to bid Non-Competitive Procurement Strategy: Exclusive Rights Comprehensive Land Claim Agreement: No Vendor Name and Address: EPPENDORF CANADA LTD. 2810 Argentia Road Unit #2 Mississauga Ontario Canada L5N8L2 Nature of Requirements:

Robotic Liquid Handler

6D063-183403/A Hall, Marlene Telephone No. - (204) 230-0147 ( ) Fax No. - (204) 983-7796 ( )

Line 1, Robotic Liquid Handler

File No: 6D063-183403/A

Title: Robotic Liquid Handler Public Works and Government Services Canada (PWGSC), on behalf of Public Health Agency of Canada (PHAC), intends to negotiate with Eppendorf North America for the provision of a Robotic Liquid Handler.

Definition of Requirement The Public Health Agency of Canada has a requirement for the supply, delivery, installation and training of one (1) Robotic Liquid Handler, with the option to purchase two (2) additional unit on or before March 31, 2021.

The robotic liquid handler required is an automation of Next-Generation Sequencing (NGS) library preparation. The library preparation kits are sourced from Illumina Inc. In the division of Zoonotic Diseases, the need of high throughput NGS is inclining through work on projects with collaborators.

The Eppendorf epMotion 5075t with NGS accessory bundle is the only "Illumina Qualified" (i.e. Illumina’s analysis indicated that libraries prepared with these methods perform comparably to those prepared manually) for all 15 different types of library prep. It ensures reproducibility with reduced chance of error, as well as decrease of "hands-on" time and increase of "walk-away" time for technicians to perform other valuable tasks. Eppendorf Canada Ltd. is the only distributer of the liquid handling station Eppendorf epMotion 5075t with NGS accessory bundle.

Security Requirement There is a security requirement associated with this requirement.

Any interested supplier must demonstrate by way of a statement of capabilities that its product meets the following mandatory requirements:

Performance / Criteria / Objectives / Specifications 1. The system must be fully enclosed and must have a safety door with pausing of operation if the door is opened avoiding injury, preventing external (humidity, temperature) interference with the method, and avoiding nuclease contaminations.

2. The system must have clean cap including HEPA filter and UV light for decontamination.

3. The system must include a worktable with a minimum of 15 positions.

a. at least one of the 15 deck positions must include a thermo-mixing module. This module must be fully integrated in the worktable and controlled via the system’s software. It must combine 2D orbital shaking with heating and cooling technology and must be able to operate while simultaneously pipetting onto other worktable positions. i. Maximum payload is 1,000 grams ii. Mixing range 300 - 2,000 rpm iii. Temperature range: 4°C from room temperature to 95°C iv. Heating rate: 5°C/min. v. Cooling rate: 3°C/min.

b. At least another one of the 15 deck positions must incorporate a Peltier based thermal element for heating or cooling of samples and reagents. This unit must be completely integrated in the worktable and must be programmed directly from the operating software. i. Temperature: 0°c to 110°C ii. Heating: from 25°C to 95°C in 8 minutes (8.75°C/min.) iii. Cooling: from 25°C to 4°C in 5 minutes (4.2°C/min.)

4. The system must provide contact free detection by measuring the light reflection of surfaces of liquid levels, of dispensing tools and of system accessories, labwares, as well as tip size and quantity. This avoids the use of more expensive, electrically conductive tips.

5. The system must operate with liquid handling tools (pipettes) that offer a pipetting range of 0.2µl to 1000µl. The system must have options to pipet this range with either a single-channel tool or an 8-channel tool. At any given time, up to 4 dispensing tools must be available for use during a program (without user intervention).

6. The system must offer air displacement pipet tools, as this reduces maintenance and complications related to a liquid back-filled syringe system.

7. The system must be able to provide contact free dispensing of samples from the pipette tip into labware, reducing the possibility of cross-contamination as well as be able to allow exact position of the pipet tip relative to liquid meniscus or labware bottom during aspiration and dispensing. This is essential for magnetic bead-based washing steps of NGS library preparations.

8. The system must provide the following pipetting precision: Coefficient of variation (%) for small volumes