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Create free accountTrade Agreement: NAFTA/AIT/Canada FTAs with Peru/Colombia/Panama Tendering Procedures: Generally only one firm has been invited to bid Attachment: None Non-Competitive Procurement Strategy: Exclusive Rights Comprehensive Land Claim Agreement: No Vendor Name and Address: CERTIFIED SCIENTIFIC INSTRUMENTS, INC. 2699 SPRING STREET REDWOOD CITY California United States 94063 Nature of Requirements: Marlene Hall Supply Specialist Telephone: 204 984-6423 E-mail: marlene.hall@pwgsc-tpsgc.gc.ca
File No.: 6D063-133001/A
TITLE: DNA Synthesizer
Public Works and Government Services Canada (PWGSC), on behalf of Public Health Agency Canada (PHAC), intends to negotiate with Certified Scientific Instruments, Inc. for the provision of a DNA Synthesizer.
DEFINITION OF REQUIREMENT
Public Health Agency Canada (PHAC) has a requirement for one (1) 3900 high throughput DNA/RNA Synthesizer system. The Genomics Core facility provides in-house DNA synthesis services to the National Microbiology Laboratory and its stakeholders. Requests from clients has expanded beyond existing capacity and a new system is required to meet the rapid response to on-going research and emergency situations such as disease outbreak. The DNA Synthesizer must be compatible with existing 3900 instrument.
Any interested supplier must demonstrate by way of a statement of capabilities that its product meets the following mandatory requirements:
PERFORMANCE CRITERIA/OBJECTIVES/SPECIFICATIONS
The Public Health of Canada requires instrumentation capable of high throughput, column-based synthesis of varying length oligonucleotides at scales of 40-, 200-, and 1000- nmol, as well as degenerate and modified oligonucleotides (e.g., dye-labeled primers and probes). the instrument must be automated DNA synthesis for at least 40 synthetic products (20 nucleotides in length) in less than 2-hours using industry-standard phosphoramidite chemistry. It must also possess inherent flexibility to synthesize sequences with lengths greater than 50-mers, and synthesize all three scales simultaneously in one run. More criteria/objective and specifications are outlined below and in the sole source (TB seven questions) document.
Mandatory operational requirements the procured high-throughput DNA synthesizer system are detailed below:
1. Must be compatible with the existing 3900 currently in operation in our laboratory.
2. To address increased throughput requirements in our Core facility and achieve our production requirements in a timely manner, the instrument must be capable of performing automated DNA synthesis in a high-throughput manner: Synthesizer must be able to synthesize at least 40 samples in parallel (i.e, not sequentially); must also be capable of making at least 20 synthetic products (20 nucleotides in length) in less than 2 hours using industry-standard phosphoramidite chemistry; must be able to produce at least 80 standard-length (20-30mers) oligonucleotides at 40- and 200- nmol scales in a single working day of 7.5 hours; must have a cycle time for one base addition to each column of less than 5 minutes. Instrument must also be capable of simultaneous 1000-nmol-scale synthesis, and of synthesizing high-quality long oligonucleotides (>50-nucleotides in length).
3. Our facility absolutely requires a column-based synthesis system, rather than a 96-well or plate-based system. System must be compatible with either CPG and polystyrene column synthesis, have color-coded display for visual confirmation of correctly loaded columns and be compatible with the existing 3900 workflow currently in use in our laboratory.
4. For optimal flexibility in a Core facility and to address multiple dye-modified oligonucleotides or DNA of different scales in a single synthesis run, System must perform fully automated (unattended) reagent delivery, synthesis and control. System also must be provided with a computer/laptop and software to enable instrument control. System must include programs and be capable of making oligonucleotides within one synthesis run at each of several synthesis scales: Small synthesis scale (40 nanomoles). Medium synthesis scale (200 nanomoles). Medium synthesis scale must use less than 2 milligrams of phosphoramidite, and less than 45 microliters of saturated 'tetrazole activator' for each nucleotide cycle. Large synthesis scale (1000 nanomoles).
5. As our Core facility supplies end users with products used for public health surveillance and diagnoses, we absolutely require System produce greater than 90% chemically authentic synthetic "quality" DNA (as measured by capillary electrophoresis, MALDI-TOF or equivalent quality assurance assay) with standard synthesis cycles (40-nmol, 200-nmol, or 1000-nmol) and standard reagents provided by the instrument's vendor.
6. For efficient operation and conservation of Core facility reagents, System must be able to predict reagent volumes necessary to complete the queued sequences; system must also track waste composition by volume and component percentage. System must also include automated reagent priming and purging feature for individual as well as system priming and purging.
7. Delivery of reagents must be accomplished by argon gas pressure; no syringes or mechanical pumps must be employed. Mechanical gas regulators must control gas pressures. Synthesizer must also be able to monitor the compressed gas pressure.
8. Synthesizer must include all program methods for cyanoethyl phosphoramidite synthesis of DNA, AND program methods for "degenerate" (e.g., software recognizes IUB codes for mixed-base site) synthetic DNA oligonucleotides at small, medium and large nanomole synthesis scales listed above. To address additional Core facility needs, System must also accept user-modified methods including novel synthesis reaction conditions (or modules).
9. Owing to space limitations in our laboratory, the synthesizer cannot exceed (with all required clearances) the footprint of 130-cm width, 85-cm depth and 120-cm height to enable placement on a small, fixed laboratory bench.
10. System must be compatible with fast-deprotection phosphoramidite chemistry, which reduces manual deprotection time requirements.
11. Reagents optimized for synthesis system performance must be available directly through the instrument's vendor and available in bulk packaging, including proprietary synthesis columns, along polystyrene supports, reagents and consumables.
12. The synthesis columns must be clearly labeled or color-coded to easily identify the coupling nucleotide (A, G, C, or T) and prevent accidental misuse of the wrong synthesis column.
13. For optimal flexibility, the instrument must be equipped with a minimum of 40 synthesis column/cartridge positions, 4 amidite stations, at least five ancillary reagent bottle positions to accommodate modified or non-standard phosphoramidites (e.g., five different modifications can be accomplished in a single run), and two additional non-amidite reagent positions for specialized synthesis (e.g, thiolating-reagent).
14. Synthesizer must include all reagent bottles, plumbing lines, fittings, connections, software, hardware, firmware, etc. to make a fully functional unit. The end user will supply synthesis chemicals, synthesis columns, and compressed gas with two-stage regulator, for reagent delivery. The supplier must include all other required system components.
16. Acetonitrile reservoir(s) with at least 8 liters capacity.
17. 'Deblock' reservoir(s) with at least 2 liters capacity.
18. 'Cap A' and 'Cap B' and 'Oxidizer' and 'Tetrazole activator' reservoir(s) of at least 900 milliliters capacity for each of these four chemical solutions.
19. Waste reservoir(s) with at least 10-liters capacity.
20. Synthesizer must include sufficient capacity to synthesize in under 2-hours (including set up and synthesis time) at least 40 independent samples simultaneously for UNATTENDED/AUTOMATIC synthesis. Instrument must be capable of reporting activities that occurred during a synthesis run. Synthesizer must automatically generate a real-time log file of synthesizer operations. For example, the software must provide a report at the conclusion of a run detailing the oligonucleotides made and their sequences.
21. The instrument must use dedicated reagent delivery lines to prevent cross contamination. Solenoid-activated valves must control flow of all reagents. Each reagent position must have a dedicated valve.
22. System must include a controlling computer with the following minimum specifications:
23. Must be a well-known business class brand-name computer, with manufacturer's warranty, and come preloaded with standard protocols. Computer must have all necessary hardware, firmware, and software for complete control of all synthesizer operations. Computer must also have the necessary hardware, firmware, and software for importing information from an Excel spreadsheet format.
24. System must come with controlling computer and software with a graphical user interface to transfer DNA sequences, functions, and cycles to Synthesizer automatically. Default protocols must be provided and allow for simultaneous synthesis of both unlabeled and labeled oligonucleotides.
25. Software must provide for synthesis operation with the following functionalities: instrument diagnostics to check status of critical system components; full-cycle editing and modification; assignment of multiple phosphoramidite bottle positions for a wide range of synthesis options; automatic import of DNA sequence information; capability for storing DNA sequence and method information; tracking and recording of runs and files for the purpose of auditing and troubleshooting. Instrument control interface must also allow immediate termination of a run, or pause at the next specified safe step.
26. Software must be able to accept electronic text-based defined format orders, created automatically, without re-entry of the sequence. For example, the software must capable of importing sequences in tab-delimited text, comma-separated text files, or Microsoft Excel spreadsheet format.
Following specifications relate to miscellaneous system procurement requirements:
27. Include on-site delivery, installation, training, and software/instrument manuals; System warranty for one year from the date of installation. This full warranty must include parts, labor and travel. The instrument must also be supported by telephone technical support, Field Applications/Sales, and instrument service. Factory-trained service engineer must be available to perform all repairs.
JUSTIFICATION FOR PRE-IDENTIFIED SUPPLIER
Certified Scientific Instruments Inc. is the only known supplier that can provide a DNA Synthesizer that meets all of the mandatory performance specifications.
TRADE AGREEMENTS
The following limited tendering reasons pertain to this requirement:
Government Contract Regulations, Part 1, Section 6(d) only one person is capable of performing the contract.
Government on Internal Trade (AIT), Article 506-12(b) where there is an absence of competition for technical reasons and the goods or services can be supplied only by a particular supplier and no alternative or substitute exists;
North American Free Trade Agreement (NAFTA), 1016.2(b) where, for works of art, or for reasons connected with the protection of patents, copyrights or other exclusive rights, or proprietary information or where there is an absence of competition for technical reasons, the goods or services can be supplied only by a particular supplier and no reasonable alternative or substitute exists; AIT, Article 506-12(b) where there is an absence of competition for technical reasons and the goods or services can be supplied only by a particular supplier and no alternative or substitute exists;
Canada-Chile Free Trade Agreement (CCFTA), Article Kbis-09 (b) where, for the works of art, or for reasons connected with the protection of patents, copyrights or other exclusive rights, or proprietary information or where there is an absence of competition for technical reason, the goods or services can be supplied only by a particular supplier and no reasonable alternative or substitute exists;
Canada-Peru Free Trade Agreement, Article 1409, 1(b) where the goods or services can be supplied only by a particular supplier and no reasonable alternative or substitute goods or services exist for any of the following reasons: (ii) the protection of patents, copyrights or other exclusive rights,
Canada-Columbia Free Trade Agreement, Article 1409, 1(b) where the goods or services can be supplied only by a particular supplier and no reasonable alternative or substitute goods or services exist for any of the following reasons: (ii) the protection of patents, copyrights or other exclusive rights,
DELIVERY DATE
The equipment must be delivered on or before March 31, 2014, FOB destination, to the Public Health Agency of Canada in Winnipeg, Manitoba, Canada.
PRE-IDENTIFIED SUPPLIER
Certified Scientific Instruments, Inc. 2699 Spring Street Redwood City, CA 94063 United States Delivery Date: Above-mentioned
You are hereby notified that the government intends to negotiate with one firm only as identified above. Should you have any questions concerning this requirement, contact the contracting officer identified above.
An Advance Contract Award Notice (ACAN) allows departments and agencies to post a notice, for no less than fifteen (15) calendar days, indicating to the supplier community that it intends to award a good, service or construction contract to a pre-identified contractor. If no other supplier submits, on or before the closing date, a Statement of Capabilities that meets the requirements set out in the ACAN, the contracting authority may then proceed with the award. However, should a Statement of Capabilities be found to meet the requirements set out in the ACAN, then the contracting authority will proceed to a full tendering process.
Suppliers who consider themselves fully qualified and available to provide the services/goods described herein, may submit a statement of capabilities in writing to the contact person identified in this Notice on or before the closing date of this Notice. The statement of capabilities must clearly demonstrate how the supplier meets the advertised requirements.
The PWGSC file number, the contracting officer's name and the closing date of the ACAN must appear on the outside of the envelope in block letters or, in the case of a facsimile transmission, on the covering page.
The Crown retains the right to negotiate with suppliers on any procurement.
Documents may be submitted in either official language of Canada.
Not stated in the notice: procurement type, procurement method, selection criteria, region of delivery, region of opportunity, expected contract period, end user
No amendments observed.
No attachments listed in the open data.
No contact listed in the open data.