Real-Time PCR Cycler

Trade Agreement: NAFTA/AIT/Canada-Peru FTA/Canada-Colombia FTA Tendering Procedures: Generally only one firm has been invited to bid Attachment: None Non-Competitive Procurement Strategy: Exclusive Rights Comprehensive Land Claim Agreement: No Vendor Name and Address: QIAGEN Inc 181 Bay Street Suite 4400 Toroto Ontario Canada M5J2T3 Nature of Requirements: Real-Time PCR Cycler

6D063-123461/A Barenz, Leanne Telephone No. - (204) 983-0506 Fax No. - (204) 983-7796

TITLE: Rotor-Gene Q 5plex

Public Works and Government Services Canada (PWGSC), on behalf of the Public Health Agency of Canada, intends to negotiate with Qiagen Inc. for the provision of the following:

The supply of one Rotor-Gene Q 5plex HRM real-time PCR cycler. This system must be complete with High Resolution Melt (HRM) analyzer with 5 channels plus HRM channel, laptop computer, software, accessories, installation and training, FOB Destination, Montreal, Quebec by March 30th, 2013.

Acting as an external reference centre for the National Microbiology Laboratory, the National Reference Centre for Parasitology (NRCP) conducts rare and unique testing for the diagnosis, surveillance, and control of unusual parasitic agents, with the objective to reduce morbidity and mortality in Canadians. The equipment being requested is to provide real time PCR detection of American and African Trypanosomiasis, leishmaniasis, filarial, malaria and other parasitic diseases.

The equipment required is essential for the timely and accurate species-specific detection of the parasitic agents under investigation (e.g., malaria, leishmania, filarial). These are diagnostic tests and patient management/treatment is informed by the results. As such it is important that the testing performed is accurate and that results are available in a timely fashion. To address the expansion of applications and enable NRCP to achieve its diagnostic and public health surveillance mandate, a tube cycler is required to perform this testing.

The system must have the following mandatory performance criteria to address NRCP's needs and mandate:

Provide high resolution melt (HRM) capability to detect a variety of genus parasites (e.g., leishmania) which have several species.

Tube cycler technology able to ensure that the temperature uniformity variation between samples is below ±0.02°C (i.e., well-to-well temperature variation).

Provide peak ramp rate (air) of 15°C/second heating and >20°C/second cooling.

Provide six separate channels to support muco-cutanuous leishmania testing.

Accommodate different volumes and multiple throughput capabilities. This flexibility is very important for the number of tests and diseases performed at the NRCP.

Provide temperature range between ambient and 99°C with a temperature calibration time of zero seconds.

Perform fast cycling without compromising performance run times (i.e, provide results in as little as 39 minutes) using the HRM application.

Provide thermal and optical uniformity of results in sensitive, precise, and fast real-time PCR analysis through tube cycler technology. This allows the tube to spin in a chamber of moving air, keeping all samples at precisely the same temperature during the fast thermal cycling, therefore eliminating sample-to-sample variations and edge-effects.

Perform up to 100 samples in a ROTOR DISC.

Offer a full audit trail to document testing and support the future implementation of a quality management system.

Able to create new excitation detection wave length combinations in order to flexibly support testing with alternate dye technologies.

Provide data analysis software. Final reporting of data from automated analysis or user defined analysis must be provided. Raw data must be exportable for validation purposes.

Ship with a computer for operations and data export and must enable operations in unattended, continuous operation with fully automated reagent delivery, incubation, detection, and data collection.

Owing to space limitations, the system cannot exceed 15 in width by 11 in height by 17 in depth and cannot not exceed 35 lbs to enable placement on a small, fixed laboratory bench.

Must be delivered on or before March 28, 2013

Qiagen Inc. has been identified as the sole vendor that achieves all of the Mandatory Performance Criteria outlined above. Qiagen is in a unique position to fulfill this requirement, as they are the owner/manufacturer and sole supplier of Rotor-Gene Q 5plex HRM platform and its consumables in the United States and Canada. Based on the above, the Crown intends to negotiate a contract with Qiagen on a sole source basis.

Name/address of sole source of product QIAGEN Inc. 181 Bay Street, Suite 4400 Toronto, Ontario M5J 2T3 www.qiagen.com

The following trade agreements are applicable to this procurement: NAFTA and AIT

Government Contract Regulations, Part 1, Section 6(d) only one supplier (person or firm) is capable of performing the contract.

Article 506.12(b) of AIT, and Article 1016: Limited Tendering Procedures of NAFTA are applicable as this requirement can be fulfilled by only one supplier.

Estimated Cost: $40,000.00 - $42,000.00 (GST not included)

Delivery Date: Above-mentioned

You are hereby notified that the government intends to negotiate with one firm only as identified above. Should you have any questions concerning this requirement, contact the contracting officer identified above.

An Advance Contract Award Notice (ACAN) allows departments and agencies to post a notice, for no less than fifteen (15) calendar days, indicating to the supplier community that it intends to award a good, service or construction contract to a pre-identified contractor. If no other supplier submits, on or before the closing date, a Statement of Capabilities that meets the requirements set out in the ACAN, the contracting authority may then proceed with the award. However, should a Statement of Capabilities be found to meet the requirements set out in the ACAN, then the contracting authority will proceed to a full tendering process.

Suppliers who consider themselves fully qualified and available to provide the services/goods described herein, may submit a statement of capabilities in writing to the contact person identified in this Notice on or before the closing date of this Notice. The statement of capabilities must clearly demonstrate how the supplier meets the advertised requirements.

The PWGSC file number, the contracting officer's name and the closing date of the ACAN must appear on the outside of the envelope in block letters or, in the case of a facsimile transmission, on the covering page.

The Crown retains the right to negotiate with suppliers on any procurement.

Documents may be submitted in either official language of Canada.