IVIS Spectrum CT Instrument Package

Trade Agreement: WTO-AGP/NAFTA/AIT/Canada-Peru FTA/Canada-Colombia FTA Tendering Procedures: Generally only one firm has been invited to bid Attachment: None Non-Competitive Procurement Strategy: Exclusive Rights Comprehensive Land Claim Agreement: No Vendor Name and Address: PERKIN ELMER (CANADA) LTD ANALYTICAL INSTRUMENTS DIVISION 330 COCHRANE DRIVE MARKHAM Ontario Canada L3R8E5 Nature of Requirements: IVIS Spectrum CT Instrument Package

6D063-122764/A Barenz, Leanne Telephone No. - (204) 983-0506 Fax No. - (204) 983-7796

Public Works and Government Services Canada (PWGSC), on behalf of the Public Health Agency of Canada (PHAC), intends to negotiate with Perkin Elmer Health Sciences Inc. for the provision of the following:

IVIS Spectrum-CT Imaging System

PHAC has a requirement for the purchase of the IVIS Spectrum-CT Imaging System from Perkin Elmer Health Sciences Inc.

The IVIS imaging system analyzes the source and strength of light emitted by bioluminescent or fluorescent molecules that are expressed in a live organism and co-registers this data with CT anatomical data to produce true quantitative 3D optical reconstructions. This particular combination of multiple modalities enables the user to visualize and quantitate 3D fluorescence and 3D bioluminescence in an entire animal or focus on one organ or only a few cells specifically. In addition, it makes possible non-invasive longitudinal studies of disease progression, by facilitating the examination of cellular response, and being able to monitor changes in gene expression patterns in live animals.

The IVIS Spectrum-CT Imaging System from Perkin Elmer Health Sciences Inc. is the only product which can meet the mandatory requirements specified below. Of these, most importantly, the IVIS Spectrum-CT Imaging System is the only instrument that offers fluorescent and bioluminescent imaging, Cerenkov luminescent imaging (for optical radiotracer imaging), 3D optical topographic reconstructions as well as integrated micro Computed Tomography (CT) imaging in a single integrated portable instrument. We require these multiple modalities to be able to over lay distinct data sets to obtain 3D fluorescence and 3D bioluminescence reconstructions which then allow us to put the optical signals obtained into an anatomic context when we perform live animal imaging to monitor cellular response and gene expression change in response to disease or treatment. Due to extreme space constraints we strictly require that this instrument have a footprint of less than 75cm x 75cm. The IVIS Spectrum-CT Imaging System from Perkin Elmer Health Sciences Inc. is the only instrument that can offer the capability required within the footprint specified.

SYSTEM REQUIREMENTS (SOLE SOURCE SPECIFICATIONS) SYSTEM DESIGN: "The system must provide the following imaging modalities in a single integrated unit/one footprint; photographic imaging, fluorescent imaging, bioluminescent imaging, Cerenkov luminescent imaging (for optical radiotracer imaging), 3D optical topography for fluorescence and bioluminescence, as well as integrated micro Computed Tomography (CT) imaging. The system must have a fully integrated design that incorporates camera, cooling system, chamber, fluorescence system, luminescence system, and micro CT system.

The system must provide integrated fast and low dose micro Computed Tomographic scanning, such that full animal scans take 10 seconds or less, and the animals are subjected to less than 10 mGrey dose.

The system must provide 3D Topographic imaging, i.e 3D surface topographic images which when processed with the proper analysis software provides diffuse tomographic reconstructions of internal sources for bioluminescent and fluorescent sources.

The system must provide automated co-registration to automatically overlay 3-dimensional bioluminescence, fluorescence, and micro Computed Tomography reconstructions.

The system must come complete with a software package for detection, quantification, analysis and storage of images taken. The software must quantify photon counts, but can also convert the data into false color images that it can overlay or co-localize on images of the animal subject such that the location of the photon sources in the subject can be precisely identified.

The system must have the ability to be able to detect down to a single bioluminescent cell non-invasively (single cell resolution).

The system must have a 5 animal capacity.

The system must have a fully integrated design that incorporates camera, cooling system, chamber, fluorescence system, luminescence system, and micro CT system.

The system must be portable.

The system must have a strategy in place for reducing auto-fluorescent background. Specifically, the system must provide instrument background subtraction via software correction, and emission based spectral unmixing for this purpose.

The system must provide a footprint equal to or smaller than 28 inches x 28 inches (72cm x 72cm)

OPTICAL TRAIN: The system must provide a sensor size of greater than or equal to 2.7cm x 2.7cm and a back illuminated Grade 1 CCD camera with a CCD operating temperature of -90C.

The system must provide for 4MP camera for high resolution imaging or binned imaging for high sensitivity imaging.

The system must provide a pixel size of 13.5um or larger on the sensor for high sensitivity imaging.

The system must provide for a 27um pixel size or larger when binned 2x2.

The system must have a minimum detectable radiance of 70 photons/s/sr/cm2.

The lens must provide at least 4 levels of magnification (1.5x, 2.5x, 5x, 8.7x).

The field of view (FOV) range must be at least 3.9cm x 3.9cm to 23cm x 23cm

The system must achieve uniform light collection at f/1 with high resolution over the entire field of view.

The system must provide a quantum efficiency of >85% at 500-700nm, and > 30% at 400-900nm.

The system must have a dark current (typical) of