Ultra-High Performance Liquid Chromatography QTRAP 6500+ System and Ion Mobility Spectrometry (UHPLC

Trade Agreement: WTO-AGP/NAFTA/AIT/Canada FTAs with Peru/Colombia/Panama Tendering Procedures: Generally only one firm has been invited to bid Non-Competitive Procurement Strategy: Exclusive Rights Comprehensive Land Claim Agreement: No Vendor Name and Address: AB Sciex LP 71 Four Valley Dr Concord Ontario Canada L4K4V8 Nature of Requirements:

Ultra-High Performance Liquid Chromatography QTRAP 6500+ System and Ion Mobility Spectrometry (UHPLC

39903-170344/A Chubey, Karen Telephone No. - (204) 291-5928 Fax No. - (204) 983-7796 Email: karen.chubey@pwgsc-tpsgc.gc.ca

Public Works and Government Services Canada (PWGSC) on behalf of the Canada Food Inspection Agency intends to negotiate with AB Sciex LP. for the supply of one ultra-high performance liquid chromatography QTRAP 6500+and ion mobility spectrometry (UHPLC-QTRAP/IMS) which has the capabilities of quantifying and identifying a large number of pesticides and other chemical contaminants, differentiating isomers, and increasing selectivity to reduce interferences from samples in a single analysis.

The required system includes an ultra-high performance liquid chromatography, hybrid triple quadrupole/linear ion trap and ion mobility spectrometry (UHPLC-QTRAP/IMS). The system must have capabilities of accurately quantifying and unambiguously identifying a large number of pesticides and other chemical contaminants, differentiating isomers, and increasing selectivity to reduce interferences at low parts-per-billion concentration levels in both positive and negative modes with fast data acquisition speed and polarity switching in a single analysis. A system from AB Sciex that includes EXION AD UHPLC, Sciex QTRAP 6500+, and SelexION+ Ion Mobility Spectrometry is identified to meet all mandatory requirements.

Criteria for assessment of the Statement of Capabilities

MinimumEssential Requirements Any interested supplier must demonstrate by way of a statement of capabilities that its product meets the following requirements:

Minimum Mandatory Capabilities/Technical/Performance Criteria/Specifications

1.1. UHPLC-QTRAP/IMS System Capabilities 1.1.1. The whole required system must include an ultra-high performance liquid chromatography, a hybrid triple quadrupole/linear ion trap, and an ion mobility spectrometry (UHPLC-QTRAP/IMS). The UHPLC-QTRAP/IMS system must have capabilities of performing MRM (multiple reaction monitoring) for quantification, carrying on enhanced product-ion spectral scan for identification, and providing an orthogonal separation by IMS for differentiating isomers in a single injection for the determination of pesticides and other chemical contaminants in food. All these must be achieved at low parts-per-billion concentration levels in both positive and negative modes with fast data acquisition speed and polarity switching in a single analysis. The mass spectrometer must be able to perform MRM and enhanced product ion spectral scan simultaneously. As MRM transitions are positively detected, the data files (i.e. product ion spectra) can be directly searched against a mass spectral library to unambiguously confirm the eluted analytes by the product ion spectral scan - simultaneous to the quantification being reported in the same injection. The UHPLC-QTRAP must be able to be coupled with an ion mobility spectrometry device, which is amenable to MRM analysis with fast dwell times, in methods with polarity switching, to perform functions of differentiating isomers and separating analytes of interest from interferences in complex sample matrices. The ion mobility device must be able to be installedand removed under 5 mins to toggle between modes quickly without venting the system.

1.1.2. The UHPLC-QTRAP must have complete capability and compatibility to import and run the existing validated API 5000 LC/MS/MS methods and compound libraries of a large number of pesticides, i.e. ~ 450, already in use in the Quality System at the Calgary Laboratory. As a part of mandatory requirements, all current UHPLC/ESI-MS/MS methods of up to 450 pesticides and libraries are required to transfer from the API5000 LC/MS/MS to UHPLC-QTRAP in less than 5 mins without retuning instrument parameters for individual pesticides while achieving the sensitivity and a dynamic range specified below.

1.1.3. UHPLC-QTRAP must deliver an increase in sensitivity of = 5 times in S/N on UHPLC/MS/MS methods already optimized on the API 5000 LC/MS/MS System for 90% of the 450 pesticides with an extended dynamic quantification range of = 3 orders of magnitude. It must provide the capability of combining positive and negativemode analysis into a single injection method with a 1 ms pause time, 1 ms dwell time and 5 ms polarity switches for a fast data acquisition to increase the method capacity. The software that comes with UHPLC-QTRAP must enable to directly access and analyse historic archived API 5000 LC/MS/MS data in the Quality System at the Calgary Laboratory.

1.2. Mass Spectrometer 1.2.1. MS type: hybrid triple quadrupole/linear ion trap LC/MS/MS mass spectrometer.

1.2.2. Supplied with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). The instrument ionization source housing must have a source housing hosting interchangeable APCI (Atmospheric Pressure Chemical Ionization) probe and ESI probes. Each probe will be coded for automatic identification by the software/firmware of the host system.

1.2.3. The source must have flow rates compatibility from 5 to 3000 µL/min, without flow splitting for ESI and 200 to 3000 µL /min for APCI.

1.2.4. Sensitivity MRM mode -positive: Reserpine 1 pg on column S/N > 510,000.

1.2.5. Sensitivity MRM mode -negative: Chloramphenicol 1 pg on column S/N > 180,000.

1.2.6. Triple quad scan speed: 12,000 Da/sec.

1.2.7. Linear ion trap scan speed: 20,000 Da/sec.

1.2.8. Polarity switching time: 5 msec, in MRM and Scheduled MRM (sMRM) modes.

1.2.9. Minimum MRM dwell time: 1 msec.

1.2.10. Triple quad mass range: (m/z) 5-2000.

1.2.11. Linear ion trap mass range: (m/z) 50-2000.

1.2.12. Triple quadrupole mass stability: 0.1 Da over 24 hours.

1.2.13. Scan types: Full scan MS and selected ion for both Q1 and Q3, product ion scan, multiple reaction monitoring (MRM), Scheduled MRM (sMRM), enhanced MS scan, enhanced product ion scan, enhanced resolution scan, MS3 scan, MRM3 scan,and TripleTrap scan modes.

1.2.14. Sensitivity EPI (enhanced product ion scan) mode: Reserpine 50 fg on column 1000 µL/min, 150-650 Da at 10,000 Da/sec, sum of product ions 195 and 174, S/N > 200 and C.V. 150 and C.V.