Cape Breton University Freshwater Mussel

1. Assess the reliability of eDNA analyses in the field (Part A). A number of environmental variables can influence the ability to detect eDNA in the environment, even in the presence of known target populations. These factors include the availability of DNA in the water column, which can be influenced by distance from the source, rate of water flow, and patterns of water movement among many other factors. In addition, eDNA may be less available seasonally due to the activity patterns of bivalves or less easily assayed due to the presence of inhibitors in the water column which can also vary seasonally. Consequently, in order to develop a robust sampling strategy, it is important to evaluate the importance of sample location, distance from source, and seasonality on the ability to detect Brook Floater using eDNA. Results from this study will inform current sampling strategy when monitoring existing populations of Brook Floater and will facilitate exploration of new habitats in the search for unknown populations of this species. 2. Establish the limits of detection and limits of quantitation using detection assays (Part A). The ability to detect environmental DNA will always be a consequence of the amount/quality of DNA present in the environment and the sensitivity of the assay to detect that DNA. It is important to establish the sensitivity of the current system according to newly proposed standards for eDNA monitoring. Results from this analysis will enable standardized reporting of our field data, facilitate comparisons across eDNA studies of Brook Floater and other bivalves, and improve interpretation of spatial or temporal variation in results. 3. Develop a molecular identification key for the ten freshwater mussel species occurring in Atlantic Canada (including Yellow lampmussel, Brook Floater and Dwarf Wedgemussel) (Part B).